apc 052  (Alomone Labs)

Journal: bioRxiv

Article Title: Differential contribution of HCN1 and HCN4 to the synchronisation of sinoatrial pacemaker cells

doi: 10.64898/2025.12.16.694405

Figure Lengend Snippet: The SAN network forms the intrinsic leading pacemaker region of the heart driven by If-conducting cells. (A) Preparation of a gelatine-inflated heart indicating the SAN region (dashed line). (B) Fluorescence imaging of an anti-HCN1 (red signal) and anti-HCN4 (green signal) immunostained whole-mount right atrial preparation of a WT heart, revealing the anatomical extension (head, body, tail region) and spatial HCN1/HCN4 channel expression profile of the SAN. (C) Isolated SAN pacemaker cell (elongated cell) during a patch clamp recording. (D) Family of current traces (If) recorded from a WT pacemaker cell of the sinoatrial node using the voltage protocol shown on the top. From a holding potential of −50 mV, a series of voltage pulses ranging from −140 to - 20 mV (duration 5 s, delta V: 15 mV) were applied. (E) Mean activation curve of native If recorded from WT SAN pacemaker cells (n=13). Additional abbreviations: CT, crista terminalis; SVC, superior vena cava.

Article Snippet: After permeabilization (0.5% Triton X100, 20% DMSO in PBS) and blocking in 5% NDS (Normal Donkey Serum), the tissue was incubated with guinea-pig polyclonal HCN1 antibody (1:500; Alomone Labs, Jerusalem, Israel) and rabbit polyclonal HCN4 antibody (1:500; Alomone Labs).

Techniques: Fluorescence, Imaging, Expressing, Isolation, Patch Clamp, Activation Assay

Journal: Frontiers in Neuroanatomy

Article Title: Transmitter and ion channel profiles of saccadic omnipause neurons and cholinergic non-omnipause neurons in human nucleus raphe interpositus

doi: 10.3389/fnana.2025.1670220

Figure Lengend Snippet: Immunoperoxidase labeling of HCN subunits in human nucleus raphe interpositus (RIP). (A) Consecutive 5 μm thick coronal paraffin sections through RIP stained for HCN1 (left) and HCN2 (right). HCN1 (left) is labeled only in OPNs, which are ensheathed by perineuronal nets (red arrows) and lacks completely in cholinergic non-OPNs (green arrows) (middle). On the other hand, HCN2 (right) immunolabeling is found in both neuron groups. (B) HCN4 immunolabeling (right) is ubiquitously found both in OPNs (red arrows) in cholinergic non-OPNs (green arrows). Scale bar represents 50 μm.

Article Snippet: HCN4 subunit was detected with polyclonal guinea pig antibody (Cat #: APC-052-GP (formerly AGP-004); RRID: AB_2340957; Alomone Labs, Jerusalem, Israel), which recognizes an intracellular epitope at the N-terminus, corresponding to amino acid residues 119–155 of human HCN4.

Techniques: Labeling, Staining, Immunolabeling

Journal: Frontiers in Neuroanatomy

Article Title: Transmitter and ion channel profiles of saccadic omnipause neurons and cholinergic non-omnipause neurons in human nucleus raphe interpositus

doi: 10.3389/fnana.2025.1670220

Figure Lengend Snippet: Immunoperoxidase labeling of HCN subunits in human nucleus raphe interpositus (RIP). (A) Consecutive 5 μm thick coronal paraffin sections through RIP stained for HCN1 (left) and HCN2 (right). HCN1 (left) is labeled only in OPNs, which are ensheathed by perineuronal nets (red arrows) and lacks completely in cholinergic non-OPNs (green arrows) (middle). On the other hand, HCN2 (right) immunolabeling is found in both neuron groups. (B) HCN4 immunolabeling (right) is ubiquitously found both in OPNs (red arrows) in cholinergic non-OPNs (green arrows). Scale bar represents 50 μm.

Article Snippet: HCN4 , Guinea Pig/Polyclonal , Hyperpolarization-activated cyclic nucleotide-gated channel 4 , Alomone Labs Jerusalem BioPark (JBP) , AB_2340957 , 1:200.

Techniques: Labeling, Staining, Immunolabeling

Journal: The Journal of Physiological Sciences : JPS

Article Title: Doxorubicin-induced sinus node dysfunction associated with mitochondria and nuclear impairment in a mouse model

doi: 10.1016/j.jphyss.2025.100047

Figure Lengend Snippet: Doxorubicin-induced cardiomyopathy modelling in mice. A. A work flow of the modelling of doxorubicin (DXB)-induce cardiomyopathy in C57BL/6 N adult male mice. Saline was injected as a vehicle (Veh) control. B. The time course of the body weight in Veh- and DXB-treated mice. While the body weight of Veh mice incrementally gained, that of DXB mice decreased. n = 6 in Veh and 10 in DXB. C. Heart weight-to-tibial length ratio in Veh- and DXB-treated mice. n = 6 in Veh and 5 in DXB. D and E. Picrosirius red-stained sections (D) and averaged fibrosis area (E) of the left ventricular myocardium in Veh- and DXB-treated mice. n = 4/group. Scale bars = 20 μm. F. Representative picrosirius red-stained sections of the SN region in Veh- or DXB-treated mice and enlarged area labelled with HCN4, a pacemaker channel as a SN marker, indicated by rectangles in picrosirius red images. Scale bars = 100 μm. G. Averaged fibrosis area of the SN region in Veh- and DXB-treated mice. n = 4/group. * p < 0.05 vs Veh, determined by unpaired t -test.

Article Snippet: After blocking with 0.1 % bovine serum albumin in PBS, the sections were incubated overnight at 4 °C with rabbit polyclonal anti-HCN4 antibodies (1:100, APC-052, Alomone Labs).

Techniques: Saline, Injection, Control, Staining, Marker

Journal: The Journal of Physiological Sciences : JPS

Article Title: Doxorubicin-induced sinus node dysfunction associated with mitochondria and nuclear impairment in a mouse model

doi: 10.1016/j.jphyss.2025.100047

Figure Lengend Snippet: Chronic effect of doxorubicin (DXB) on expression levels of genes responsible for pacemaking. A and B. Gene expression levels of HCN4 pacemaker channel and Ca 2+ regulators in the sinus node (A) and the right atrial myocardium (B) in vehicle (Veh)- and DXB-treated mice. n = 6 in SN, n = 3 in right atrium, * p < 0.05 vs Veh determined by unpaired t -test.

Article Snippet: After blocking with 0.1 % bovine serum albumin in PBS, the sections were incubated overnight at 4 °C with rabbit polyclonal anti-HCN4 antibodies (1:100, APC-052, Alomone Labs).

Techniques: Expressing, Gene Expression